{"created":"2023-06-19T10:05:31.789705+00:00","id":15059,"links":{},"metadata":{"_buckets":{"deposit":"ee1df7c2-9ed7-4d9a-9bf3-d68744b549b1"},"_deposit":{"created_by":26,"id":"15059","owners":[26],"pid":{"revision_id":0,"type":"depid","value":"15059"},"status":"published"},"_oai":{"id":"oai:kwmw.repo.nii.ac.jp:00015059","sets":["7:368:369"]},"author_link":["66252","66253","66254","66255"],"control_number":"15059","item_1_alternative_title_20":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Measurement of Short-Chain Fatty Acid Metabolism of Caenorhabditis elegans Using 13C Breath Test","subitem_alternative_title_language":"en"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"98","bibliographicPageStart":"91","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"川崎医療福祉学会誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三浦, 紀称嗣","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"66252","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮田, 富弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"66253","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"66254","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MIURA, Kiyoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"66255","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MIYADA, Tomihiro"}]}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15112/00014979","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_23":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"川崎医療福祉学会"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10375470","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-4605","subitem_source_identifier_type":"PISSN"}]},"item_1_text_22":{"attribute_name":"その他(別言語)の雑誌名","attribute_value_mlt":[{"subitem_text_value":"Kawasaki medical welfare journal"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"川崎医療福祉大学大学院医療技術学研究科健康科学専攻"},{"subitem_text_value":"川崎医療福祉大学大学院医療技術学研究科健康科学専攻"}]},"item_1_textarea_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_value":"本研究では，短鎖脂肪酸であるプロピオン酸（Propionate：PA）の13C 同位体（[13C]PA）を投与した線虫Caenorhabditis elegans （C. elegans ）に13C 呼気分析法を応用することで，C. elegans におけるPA の代謝能を測定する13CO2ガス分析法を開発した．線虫に[13C]PA を投与し，自作の13CO2ガス分析装置に線虫を移して培養した後に装置内の空気を回収し13CO2/12CO2比を測定した．装置から回収された空気からは13CO2の出現が確認された．また，PA を投与した線虫においてPA 代謝関連遺伝子acdh-1 の発現が有意に増加した．これらの結果から，13CO2ガス分析で確認された13CO2の出現は，投与した[13C]PA を線虫が摂取し，消化管での吸収，代謝を経て空気中に排出されたCO2の濃度を反映していると考えられる．したがって，ヒトと類似したPA 代謝経路を有するC. elegans のPA 代謝能について，13CO2ガス分析法を用いることで比較的簡便に非侵襲的かつ定量的に測定が可能であることが示唆された．本研究で開発した13CO2ガス分析法は，短鎖脂肪酸研究において有用な分析ツールの一つになると考えられる．"}]},"item_1_textarea_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"In this study, we developed 13CO2 gas analysis to measure the metabolic capacity of propionate (PA) in Caenorhabditis elegans (C. elegans) using 13C breath analysis. 13C-labeled PA ([13C]PA) was administered to the worms, and after transferring to a 13CO2 gas analyzer and culturing, the air in the analyzer was collected and the 13CO2/12CO2 ratio was measured. The appearance of 13CO2 was confirmed in the air collected from the analyzer. In addition, the expression of the PA metabolism-related gene acdh-1 was significantly increased from the C. elegans to which PA was administered. These results suggest that the appearance of 13CO2 confirmed using 13CO2 gas analysis reflects the concentration of CO2 excreted into the air through absorption and metabolism in the gastrointestinal tract after ingesting the administered [13C] PA by worms. Therefore, it was suggested that the PA metabolic capacity of C. elegans , which has a PA metabolic pathway similar to that of humans, can be measured by using the 13CO2 gas analysis. This analysis can contribute to further understanding of the physiological effects of short-chain fatty acids."}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-25"}],"displaytype":"detail","filename":"11_三浦紀称嗣.pdf","filesize":[{"value":"522.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmw.repo.nii.ac.jp/record/15059/files/11_三浦紀称嗣.pdf"},"version_id":"aecd9915-8133-4218-a05b-134f0b363579"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Caenorhabditis elegans ，short-chain fatty acid，propionate metabolism，13CO2 gas analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"13C呼気分析法を応用した線虫 Caenorhabditis elegans の短鎖脂肪酸代謝能の測定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"13C呼気分析法を応用した線虫 Caenorhabditis elegans の短鎖脂肪酸代謝能の測定","subitem_title_language":"ja"}]},"item_type_id":"1","owner":"26","path":["369"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-11-25"},"publish_date":"2022-11-25","publish_status":"0","recid":"15059","relation_version_is_last":true,"title":["13C呼気分析法を応用した線虫 Caenorhabditis elegans の短鎖脂肪酸代謝能の測定"],"weko_creator_id":"26","weko_shared_id":-1},"updated":"2024-01-10T02:01:09.760179+00:00"}