{"created":"2023-06-19T10:04:59.000466+00:00","id":14235,"links":{},"metadata":{"_buckets":{"deposit":"8038cbf0-06ab-425e-aae3-f6c39e749708"},"_deposit":{"created_by":18,"id":"14235","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"14235"},"status":"published"},"_oai":{"id":"oai:kwmw.repo.nii.ac.jp:00014235","sets":["7:257:258"]},"author_link":["63154","63155","63152","63153","63157","63156"],"item_1_alternative_title_20":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Effect of Dinitrofluorobenzene on Contact Hypersensitivity in Mice"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"119","bibliographicPageStart":"113","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"川崎医療福祉学会誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"烏, 烏日娜"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"武政, 睦子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長野, 隆男"}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"WU, Wuruna"}]},{"nameIdentifiers":[{}],"names":[{"name":"TAKEMASA, Mutsuko"}]},{"nameIdentifiers":[{}],"names":[{"name":"NAGANO, Takao"}]}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15112/00014197","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_23":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"川崎医療福祉学会"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10375470","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-4605","subitem_source_identifier_type":"ISSN"}]},"item_1_text_22":{"attribute_name":"その他(別言語)の雑誌名","attribute_value_mlt":[{"subitem_text_value":"Kawasaki medical welfare journal"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"川崎医療福祉大学大学院医療技術学研究科健康科学専攻"},{"subitem_text_value":"川崎医療福祉大学医療技術学部臨床栄養学科"},{"subitem_text_value":"川崎医療福祉大学医療技術学部臨床栄養学科"}]},"item_1_textarea_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_value":"　接触過敏症（CHS）は，ヒトのアレルギー性接触皮膚炎の研究によく利用されるモデル動物実験系である．本研究では，CHS モデルマウスの耳介にチャレンジするジニトロフルオロベンゼン（DNFB）の濃度と回数の影響について検討した. まず，耳介の腫れを調べた結果，0.15％と0.3％ DNFB では2回のチャレンジで，0.5％ DNFB では1回のチャレンジで評価することが望ましいと考えられた．次に，耳介組織をヘマトキシリン・エオジン（HE）と免疫組織化学（IHC）を用いて染色した．IHCでは，抗Gr-1抗体を使用して耳介組織に浸潤した炎症細胞を染色した．その結果，HE 染色では0.15％と0.3％ DNFB 群で耳介組織の腫れに差は見られなかった．一方，IHC 染色では，0.3％ DNFB 群は0.15％DNFB 群と比べて耳介組織に浸潤した炎症細胞の数に有意な増加がみられた．従って，抗Gr-1抗体を使用したIHC 染色は，CHS 評価において有用な研究方法と考えられた．"}]},"item_1_textarea_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"　Contact hypersensitivity (CHS) is frequently used an experimental model system for human allergic contact dermatitis. In the present study, we investigated the effects of the concentration of dinitrofluorobenzene (DNFB) and the number of DNFB-challenge to evaluate CHS responses. The results of the ear swelling showed that one challenge for 0.5% DNFB and two times challenges for 0.15% and 0.3% DNFB were recommended. Mouse ear tissue was stained by hematoxylin and eosin (HE) and immunohistochemistry (IHC). IHC was conducted using anti-Gr-1 antibody which stained infiltrated inflammatory cells into the skin. The extent of the ear swelling was not different between 0.15% and 0.3% DNFB-challenged mice in HE stained tissue. In contrast, the number of infiltrated cells into the skin of 0.3% DNFB-challenged mice was significantly more than those of 0.15% DNFB-challenged mice in IHC stained tissue. These results suggest that IHC is a useful method to evaluate the CHS responses."}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-12-21"}],"displaytype":"detail","filename":"12　烏烏日娜.pdf","filesize":[{"value":"849.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmw.repo.nii.ac.jp/record/14235/files/12　烏烏日娜.pdf"},"version_id":"3cdfd621-1343-4c9b-a1ef-aae03ae224e0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"contact hypersensitivity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dinitrofluorobenzene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ear swelling","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immunohistochemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"接触過敏症モデル動物実験系に与えるジニトロフルオロベンゼンの影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"接触過敏症モデル動物実験系に与えるジニトロフルオロベンゼンの影響"}]},"item_type_id":"1","owner":"18","path":["258"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-12-21"},"publish_date":"2015-12-21","publish_status":"0","recid":"14235","relation_version_is_last":true,"title":["接触過敏症モデル動物実験系に与えるジニトロフルオロベンゼンの影響"],"weko_creator_id":"18","weko_shared_id":18},"updated":"2023-06-19T10:34:28.089390+00:00"}