{"created":"2023-06-19T10:04:04.311135+00:00","id":12741,"links":{},"metadata":{"_buckets":{"deposit":"7e09cefd-01ee-4449-9906-656101703348"},"_deposit":{"created_by":17,"id":"12741","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"12741"},"status":"published"},"_oai":{"id":"oai:kwmw.repo.nii.ac.jp:00012741","sets":["7:23:48"]},"author_link":["57528","57524","57525","57526","57529","57527"],"item_1_alternative_title_20":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Determination of 2-Thiothiazolidine-4-carboxylic Acid in Brassica Vegetables using High-performance Capillary Electrophoresis : Comparison with High-Performance Liquid Chromatography"}]},"item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"アブラナカ ヤサイチュウ 2 チオチアゾリジン 4 カルボンサン TTCA ノ キャピラリー デンキ エイドウホウ ニヨル ソクテイ"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"144","bibliographicPageStart":"135","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"川崎医療福祉学会誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"楊井, 理恵"}],"nameIdentifiers":[{"nameIdentifier":"57524","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河辺, 聡子"}],"nameIdentifiers":[{"nameIdentifier":"57525","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤井, 俊子"}],"nameIdentifiers":[{"nameIdentifier":"57526","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_17":{"attribute_name":"URL","attribute_value_mlt":[{"subitem_description":"http://www.kawasaki-m.ac.jp/soc/mw/journal/jp/2004-j14-1/j21_yanai.pdf","subitem_description_type":"Other"}]},"item_1_description_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_1_description_3":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Original Paper","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"57527","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yanai, Rie"}]},{"nameIdentifiers":[{"nameIdentifier":"57528","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kawabe, Satoko"}]},{"nameIdentifiers":[{"nameIdentifier":"57529","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Fujii, Toshiko"}]}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15112/00012730","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_23":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"川崎医療福祉学会"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10375470","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-4605","subitem_source_identifier_type":"ISSN"}]},"item_1_text_22":{"attribute_name":"その他(別言語)の雑誌名","attribute_value_mlt":[{"subitem_text_value":"Kawasaki medical welfare journal"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"山口県立大学生活科学部栄養学科"},{"subitem_text_value":"川崎医療短期大学介護福祉科"},{"subitem_text_value":"川崎医療福祉大学医療技術学部臨床栄養学科"}]},"item_1_textarea_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_value":"キャピラリーゾーン電気泳動法(CZE)とミセル動電クロマトグラフ法(MEKC)を用いて,アブラナ科野菜のキャベツとブロッコリー中の2-チオチアゾリジン-4-カルボン酸(TTCA)を簡便,かつ,迅速に分析する方法について記述した.CZE用泳動溶液は15mMβ-シクロデキストリン入りの20mM四ほう酸ナトリウム溶液を,MEKC用はCZE用泳動溶液に界面活性剤として50mMのドデシル硫酸ナトリウムを加えた泳動溶液を用いた.分析条件は,以下の通りである.キャピラリーは溶融シリカ製,内径75μm × 長さ50cmのものを用い,25℃,15kVの定電圧で測定した.試料注入量は約24nLである.測定波長は273nmを用いた.TTCAは,CZEでは6.1分,MEKCでは7.1分に移行した.TTCAの検出限界はCZEとMEKCのいずれも約4μg/Lで,検量線は0.25～10mg/Lの濃度範囲で良好な直線性を示した.本研究をアブラナ科野菜のキャベツとブロッコリー中のTTCA分析に応用した結果,CZEはキャベツとブロッコリー中のTTCAの定性法として有用であるが,MEKCではキャベツとブロッコリー中のTTCA濃度の測定値とHPLCによる測定値とがよく一致することが認められた.アブラナ科野菜中のTTCA測定法について比較すると,MEKCは,HPLCに比べて分析時間が短いこと,また,野菜からTTCAを水抽出後に適宜水で希釈した試料においても定量できるので,エーテル抽出に要する前処理が省けるなどの利点が認められた."}]},"item_1_textarea_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"A simple and rapid method is described using capillary zone electrophoresis (CZE), and micellar electrokinetic capillary chromatography (MEKC) for the qualitative and quantitative analysis of 2-thiothiazolidine-4-carboxylic acid (TTCA) in Brassica vegetables. Buffers used were a 20 mM sodium tetraborate buffer with 15 mM β-cyclodextrin for CZE and the same buffer containing 50 mM sodium dodecyl sulfate as an ionic surfactant for MEKC. Analytical conditions were as follows: A fused-silica capillary 75 μm I.D.×50 cm length was used. The temperature was set at 25℃ and a constant voltage of 15 kV was applied. The injection volume was ca. 24 nL and the UV detector was set at a wavelength of 273 nm. Mean migration times in minutes were 6.1 and 7.1 using CZE and MEKC, respectively. The detection limits of TTCA were about 4 μg/L in both methods. Liner relationships were found between peak areas and TTCA concentrations from 0.25 to 10.0 mg/L. The present method was applied to the analysis of TTCA in Brassica vegetables (cabbage and broccoli). The MEKC method showed a good correlation with the high-performance liquid chromatography (HPLC) method for the quantitative analysis of TTCA concentrations in cabbage and broccoli, whereas the CZE method was useful as a qualitative measure. A comparison of the two methods showed that MEKC was superior to HPLC because total analysis time was shorter, and the ether extraction procedure of TTCA in the vegetables could be saved."}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-01-31"}],"displaytype":"detail","filename":"j21_yanai.pdf","filesize":[{"value":"322.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmw.repo.nii.ac.jp/record/12741/files/j21_yanai.pdf"},"version_id":"064299f9-4080-42cf-99c7-7f26634d8ab0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"2-Thiothiazolidine-4-carboxylic acid (TTCA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Brassica vegetables","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"capillary zone electrokinetic (CZE)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"micellar electrokinetic capillary chromatography (MEKC)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high-performance liquid chromatography (HPLC)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アブラナ科野菜中2-チオチアゾリジン-4-カルボン酸(TTCA)のキャピラリー電気泳動法による測定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アブラナ科野菜中2-チオチアゾリジン-4-カルボン酸(TTCA)のキャピラリー電気泳動法による測定"}]},"item_type_id":"1","owner":"17","path":["48"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-01-01"},"publish_date":"2004-01-01","publish_status":"0","recid":"12741","relation_version_is_last":true,"title":["アブラナ科野菜中2-チオチアゾリジン-4-カルボン酸(TTCA)のキャピラリー電気泳動法による測定"],"weko_creator_id":"17","weko_shared_id":17},"updated":"2023-06-19T10:52:17.700379+00:00"}