{"created":"2023-06-19T10:03:52.512122+00:00","id":12488,"links":{},"metadata":{"_buckets":{"deposit":"83d58ce8-eedd-45ee-a9bf-61e68775ed90"},"_deposit":{"created_by":17,"id":"12488","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"12488"},"status":"published"},"_oai":{"id":"oai:kwmw.repo.nii.ac.jp:00012488","sets":["7:18:58"]},"author_link":["55607","55609","55608","55606"],"item_1_alternative_title_20":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"<Original Paper>Properties of Protease Produced by Staphylococcus epidermidis"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-06-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"111","bibliographicPageStart":"103","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"川崎医療福祉学会誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"二宮, 健司"}],"nameIdentifiers":[{"nameIdentifier":"55606","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"美祢, 弘子"}],"nameIdentifiers":[{"nameIdentifier":"55607","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"55608","nameIdentifierScheme":"WEKO"}],"names":[{"name":"NINOMIYA, Kenji"}]},{"nameIdentifiers":[{"nameIdentifier":"55609","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MINE, Hiroko"}]}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15112/00012477","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_23":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"川崎医療福祉学会"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10375470","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-4605","subitem_source_identifier_type":"ISSN"}]},"item_1_text_22":{"attribute_name":"その他(別言語)の雑誌名","attribute_value_mlt":[{"subitem_text_value":"Kawasaki medical welfare journal"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"川崎医療福祉大学院医療技術学研究科臨床栄養学専攻"},{"subitem_text_value":"川崎医療福祉大学医療技術学部臨床栄養学科"}]},"item_1_textarea_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_value":"健康なヒトの右手人差し指から90株のStaphylococcus epidermidisを分離した.CaCl_2-カゼイン平板法によってprotease産生量の測定をおこなったところ, 90株中84株(93.3%)が活性を示した.活性の最も強かったUNO67株を選び以後の実験に使用した.UNO67株の培養上清をprotease液として生化学的性状の検査をおこなった.まず最初にproteaseの耐熱性テストをおこなった.70℃1C分間の加熱処理でprotease活性が完全に失われることから, 本proteaseは易熱性であると判定した.次にproteaseのpH依存性を調べたところ至適pHは広い中性域であった(pH5～9).Protease活性はEDTA, phosphoramidon, phenanthrolineおよびHgCl_2によって阻害されNEM, pepstatinAおよびSBTIでは阻害を受けないことから, 本proteaseはmetaroproteaseであると判定した.定性的な金属インディケーター(メルコクアント)により含有金属種を検討したところAl, Co, Cu, Fe, MnおよびPbに対する反応は陰性であったがZnに対する反応だけは陽性であった.透析したUNO67株の培養上清をDEAE-Sephadexを用いて精製した.Protease活性は0.2MI KClで溶出される画分に局在していたため, この画分を凍結乾燥し精製proteaseとした, ProteaseはSDS-PAGEにより一本のタンパク質バンドとなり, 精製されていることが示された."}]},"item_1_textarea_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Ninety strains of Staphylococcus epidermidis were isolated from the right forefingers of healthy people. The amount of protease activity produced by those 90 strains was measured by the calcium-casein agar plate method. Eighty-four out of 90 strains tested (93.3%) showed protease activity, and the most active strain, UN067,was selected for use in future experiments. Some biochemical properties were examined by using the culture supernatant of the UN067 strain as a protease solution. The heat tolerance was tested first. Protease activity completely disappeared when it was heated for 10 minutes at 70-C. As a result, this protease was classified as a heat labile enzyme. Next, pH dependency was measured and it was shown that the optimal pH had a wide neutral range (pH 5 to 9). Protease activity was inhibited by EDTA, phosphoramidon, phenanthroline and HgCl_2 but NEM, pepstatin A and SBTI had no effect. This indicated that the protease was a metaroprotease. According to the quantitative metal indicator test (mercoquant), the reactions to Al, Co, Cu, Fe, Mn and Pb were negative while the reaction of Zn was positive. A dialyzed crude preparation of the protease was purified by DEAE Sephadex chromatography and enzyme activity was found to be localized in the 0.1 M KC1 eluted fraction. A purified powder was prepared by freeze drying the active fraction. SDS-PAGE of the powder resulted in a single protein band which suggests that it was pure."}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-01-31"}],"displaytype":"detail","filename":"KJ00000192799.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmw.repo.nii.ac.jp/record/12488/files/KJ00000192799.pdf"},"version_id":"a5113d0b-33bd-4d11-af21-37841fc50ef8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Staphylococcus epidermidis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PROTEASE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RESIDENT FLORA CNS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ZINC","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<原著>Staphylococcus epidermidisの産生するproteaseの性状について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<原著>Staphylococcus epidermidisの産生するproteaseの性状について"}]},"item_type_id":"1","owner":"17","path":["58"],"pubdate":{"attribute_name":"公開日","attribute_value":"1999-06-25"},"publish_date":"1999-06-25","publish_status":"0","recid":"12488","relation_version_is_last":true,"title":["<原著>Staphylococcus epidermidisの産生するproteaseの性状について"],"weko_creator_id":"17","weko_shared_id":17},"updated":"2023-06-19T10:58:17.924812+00:00"}