{"created":"2023-06-19T10:03:44.052715+00:00","id":12314,"links":{},"metadata":{"_buckets":{"deposit":"6e64da74-8cf5-40e1-9943-9d3b4c86eaa1"},"_deposit":{"created_by":17,"id":"12314","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"12314"},"status":"published"},"_oai":{"id":"oai:kwmw.repo.nii.ac.jp:00012314","sets":["7:13:67"]},"author_link":["54273","54274","54272","54276","54275","54271"],"item_1_alternative_title_20":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Partial Purification and Examinations on the Properties of Protease produced by Bacillus subtilis MT-l9"}]},"item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"BACILLUS SUBTILIS MT-19 カブ ノ サンセイスル プロテアーゼ ノ ブブン セイセイ ト セイジョウ ケンサ"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"152","bibliographicPageStart":"145","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"川崎医療福祉学会誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高取, 美保"}],"nameIdentifiers":[{"nameIdentifier":"54271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"口羽, 由記"}],"nameIdentifiers":[{"nameIdentifier":"54272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"美祢, 弘子"}],"nameIdentifiers":[{"nameIdentifier":"54273","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54274","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takatori, Miho"}]},{"nameIdentifiers":[{"nameIdentifier":"54275","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kuchiba, Yuki"}]},{"nameIdentifiers":[{"nameIdentifier":"54276","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Mine, Hiroko"}]}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15112/00012303","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_23":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"川崎医療福祉学会"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10375470","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-4605","subitem_source_identifier_type":"ISSN"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Nutrition, Kusaka Hospital"},{"subitem_text_language":"en","subitem_text_value":"Department of Public Health, Faculty of Medicine, Okayama University"},{"subitem_text_language":"en","subitem_text_value":"Department of Clinical Nutrition, Faculty of Medical Professions, Kawasaki University of Medical Welfare"}]},"item_1_text_22":{"attribute_name":"その他(別言語)の雑誌名","attribute_value_mlt":[{"subitem_text_value":"Kawasaki medical welfare journal"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"草加病院栄養給食部"},{"subitem_text_value":"岡山大学医学部公衆衛生学教室"},{"subitem_text_value":"川崎医療福祉大学医療技術学部臨床栄養学科"}]},"item_1_textarea_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_value":"空中より分離したBacillus subtilis MT-19株は透析カゼイン酵素加水分解物を単一の窒素源とする合成培地中にプロテアーゼを産生する.培養上清を蒸留水に対して透析した後, 凍結乾燥してプロテアーゼの粗標品を得た.粗プロテアーゼをDEAE-Sephacelを用いたクロマトグラフィーにより部分精製して各種の生化学的性状の検査をおこなった.一連の実験のプロテアーゼ活性はS-2586を酵素の合成基質として測定した.この結果得られたプロテアーゼは分子量が20,000(SDS-PAGE後カゼイン寒天転写法による)～21,000(SephadexG-75を用いたゲル濾過法による)と計算された.また, このプロテアーゼの至適pHは9であり, メタロプロテアーゼの阻害剤であるEDTAによってのみ活性化が低下する事が明確に示された.さらに, プロテアーゼ活性は, 80℃で10分間または70℃で40分間の加熱処理により完全に消失した."}]},"item_1_textarea_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Isolated airborne Bacillus subtilis MT-19 excreted protease into a chemically defined culture medium containing dialyzed enzymatic hydrolyzate of casein as a sole source of nitrogen. The supernatant of the culture medium was lyophilized after dialysis against distilled water. Crude protease was then purified through chromatography on DEAE-Sephacel and several chemical properties were studied using the partially purified sample. Protease activity was assayed using S-2586 as synthetic enzyme substrate. Molecular weight was estimated to be 20,000 by the method of casein-agar enzymography after SDS-PAGE and 21,000 by the method of gel-filtration using sephadex G-75.It was clearly shown that the protease had an optimal activity at pH 9 and was specifically inhibited by the metal protease inhibitor, EDTA. The activity completely disappeared after heating 10 minutes at 80℃ or 40 minutes at 70℃."}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-01-31"}],"displaytype":"detail","filename":"KJ00000192577.pdf","filesize":[{"value":"487.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmw.repo.nii.ac.jp/record/12314/files/KJ00000192577.pdf"},"version_id":"f0f3fac1-f6c8-431b-b50f-eefa8fe40230"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bacillus subtilis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metal protease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"isolated bacillus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"casein agar plate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bacillus subtilis MT-19 株の産生するプロテアーゼの部分精製と性状検査","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bacillus subtilis MT-19 株の産生するプロテアーゼの部分精製と性状検査"}]},"item_type_id":"1","owner":"17","path":["67"],"pubdate":{"attribute_name":"公開日","attribute_value":"1995-01-01"},"publish_date":"1995-01-01","publish_status":"0","recid":"12314","relation_version_is_last":true,"title":["Bacillus subtilis MT-19 株の産生するプロテアーゼの部分精製と性状検査"],"weko_creator_id":"17","weko_shared_id":17},"updated":"2023-06-19T11:02:23.503426+00:00"}