{"created":"2023-06-19T10:03:40.130874+00:00","id":12204,"links":{},"metadata":{"_buckets":{"deposit":"6c5c85b2-cd4b-4f48-a185-1f700436d525"},"_deposit":{"created_by":17,"id":"12204","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"12204"},"status":"published"},"_oai":{"id":"oai:kwmw.repo.nii.ac.jp:00012204","sets":["7:12:68"]},"author_link":["53691","53695","53693","53694","53692","53696"],"item_1_alternative_title_20":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"A Simple Detection Method of Candida albicans Aspartic proteinase"}]},"item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"CANDIDA ALBICANS ASPARTIC PROTEINASE ノ カンイ ケンシュツホウ"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"80","bibliographicPageStart":"75","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"川崎医療福祉学会誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"美祢, 弘子"}],"nameIdentifiers":[{"nameIdentifier":"53691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"星加, 和徳"}],"nameIdentifiers":[{"nameIdentifier":"53692","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"津島, 弘文"}],"nameIdentifiers":[{"nameIdentifier":"53693","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_full_name_7":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53694","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Mine, Hiroko"}]},{"nameIdentifiers":[{"nameIdentifier":"53695","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hoshika, Kazunori"}]},{"nameIdentifiers":[{"nameIdentifier":"53696","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tsushima, Hirofumi"}]}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15112/00012193","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_23":{"attribute_name":"公開者","attribute_value_mlt":[{"subitem_publisher":"川崎医療福祉学会"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10375470","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-4605","subitem_source_identifier_type":"ISSN"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Clinical Nutrition, Faculty of Medical Professions, Kawasaki University of Medical Welfare"},{"subitem_text_language":"en","subitem_text_value":"The Second Division of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School"},{"subitem_text_language":"en","subitem_text_value":"Laboratory for Medical Biology Tsushima Clinic"}]},"item_1_text_22":{"attribute_name":"その他(別言語)の雑誌名","attribute_value_mlt":[{"subitem_text_value":"Kawasaki medical welfare journal"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"川崎医療福祉大学医療技術学部臨床栄養学科"},{"subitem_text_value":"川崎医科大学内科消化器部門 II"},{"subitem_text_value":"津島メディカルバイオロジー研究所"}]},"item_1_textarea_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_textarea_value":"患者の舌部白苔より, 12株のCandidaを単離し, アピCオクサノグラムを利用して菌種の同定をおこなった。6株がCandida albicans, 2株がCandida tropicalis, 2株がCandida parapsilosis, 2株がCandida gulliermondiiと同定された.こられ単離, 同定した12株のCandidaをヘモグロビン平板培地に移植培養した時に増殖菌帯の周囲に形成されるロハの有無により酸性プロテアーゼ産生を簡易に検出できた。C.albicansは6株すべて酸性プロテアーゼを産生したが, C.albicans以外の6株の増殖菌帯の周囲にはロハが形成されなかった.C.albicansをヒト毛髪ケラチン培地で培養し, 培養上清を一連のプロテアーゼインヒビターとともにヘモグロビン平板培地上のホールに入れて培養し酵素の性状を調べた.酵素活性はPepstaモinによってのみ阻害された.この結果は検出した酵素がCandida Aspartic Proteinase(CAP)であることを示している."}]},"item_1_textarea_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"Twelve Candida strains were isolated from fur on the patient's tongues and their species were determined by using API-C-AUXANOGRAM. Six Candida strains were identified as Candida albicans, two as Candida tropicalis and two as Candida parapsilosis and two were identified as Candida gulliermondii. Those isolated and identified Candida strains were inoculated on hemoglobin agar plates and acid proteinase production was simply detected by the existence of halo around their developed streak. All of the six C. albicans strains produced acid proteinase but the other six Candida strains did't form any detectable halo around their streak. C. albicans strains were cultured in the human hair-keratin medium and the culture fluid was applied into the holes with a series of protease inhibitors on hemoglobin agar plates to examine their enzymatic properties. The proteolytic activity was specifically inhibited only by pepstatin, which clearly showed that this proteolytic enzyme was Candida aspartic proteinase (CAP)."}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-01-31"}],"displaytype":"detail","filename":"KJ00000192471.pdf","filesize":[{"value":"434.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://kwmw.repo.nii.ac.jp/record/12204/files/KJ00000192471.pdf"},"version_id":"7cab55e6-de29-47bf-92a8-67dd478af98a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Candida albicans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Candida aspartic proteinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hemoglobin agar plate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Candida albicans Aspartic Proteinaseの簡易検出法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Candida albicans Aspartic Proteinaseの簡易検出法"}]},"item_type_id":"1","owner":"17","path":["68"],"pubdate":{"attribute_name":"公開日","attribute_value":"1994-01-01"},"publish_date":"1994-01-01","publish_status":"0","recid":"12204","relation_version_is_last":true,"title":["Candida albicans Aspartic Proteinaseの簡易検出法"],"weko_creator_id":"17","weko_shared_id":17},"updated":"2023-06-19T11:04:48.107177+00:00"}